GLUCOSE NEED TO BOOK Molymods (C,H,O, each group

GLUCOSE NEED TO BOOK Molymods (C,H,O, each group

GLUCOSE NEED TO BOOK Molymods (C,H,O, each group will need 6 carbon, 12 hydrogen & 6 oxygen) Practical Activity 19 (PAG 9) Repro sheet for Practical Activity 19 LEARNING OBJECTIVE Know about monosaccharides

SUCCESS CRITERIA Compare alpha and beta glucose molecules Describe the ring structure and properties of glucose and ribose Compare hexose and pentose monosaccharides Explain the formation and breakage of

glycosidic bonds Carry out benedicts test for reducing sugars WHAT IS THE FORMULAE OF GLUCOSE? C6H12O6 - Compare alpha and beta glucose molecules ANSWERS What are the differences?

- Compare alpha and beta glucose molecules HEXOSE MONOSACCHARIDE: GLUCOSE Remember you can check youve done it right by counting the number of C,H,Os and that they all have the right number of bonds - Compare alpha and beta glucose molecules MOLECULAR DIAGRAM RIBOSE

- Describe the ring structure and properties of glucose and ribose - Compare hexose and pentose monosaccharides - Describe the ring structure and properties of glucose and ribose - Compare hexose and pentose monosaccharides WHAT ARE THE DIFFERENCES BETWEEN A PENTOSE & HEXOSE MONOSACCHARIDES? Hexose = 6 carbon atoms Pentose = 5 carbon atoms

GLYCOSIDIC BONDS - Explain the formation and breakage of glycosidic bonds Alpha glucose Maltose Amylose Monosaccharide Disaccharide Polysaccharide

Glycosidic between two glucose a condensation bonds form molecules by reaction - Explain the formation and breakage of glycosidic bonds 1-4 GLYCOSIDIC BOND FORMATION

- Explain the formation and breakage of glycosidic bonds MAKING DISACCHARIDES Sucrose Fructose Lactose Glucose

+ Glucose + Galactose Maltose Glucose + Glucose Maltose Sucrose PAG9 Qualitative Testing: Food Tests

You will need in your lab book: A method for one of the food tests Results tables for all the food tests Photos of all the food tests To be completed by the end of lesson 6 biological molecules - Carry out benedicts test for reducing sugars PRACTICAL: ACTIVITY 19

What is the colours of the positive and negative test for: Starch? Glucose? Practical Skills: Evaluative Analysing experiment - Carry out benedicts test for reducing sugars REDUCING SUGARS (BENEDICTS TEST) PAG9 1. 2. 3.

Add sample to a test tube Add Benedicts solution and put in a water bath, heat sample. Positive test will show a brick red and a range of colours between depending on amount. - Carry out benedicts test for reducing sugars NON-REDUCING SUGARS (BENEDICTS TEST) PAG9 1. 2. 3.

4. 5. Add sample to a test tube Add HCl, heat for 5 mins Sodium hydrogen carbonate to neutralise Add Benedicts solution and put in a water bath, heat sample. Positive test will show a brick red and a range of colours between depending on amount. - Carry out benedicts test for reducing sugars STARCH (IODINE

SOLUTION) PAG9 1. 2. 3. Add sample to a well in a spotting tile Add iodine solution to the well Positive result will show a colour change from orange/brown to blue/black Testing for Description Negative Result

Positive Results Starch Add iodine Brown Blue/black Reducing Sugar Add Benedicts

Heat to 80oC Blue Orange/Red Non-reducing sugar Negative to RS Boil in acid Neutralise Add Benedicts Heat to 80oC

Blue Orange/Red PLENARY: QUICK QUIZ - Compare alpha and beta glucose molecules - Describe the ring structure and properties of glucose and ribose - Compare hexose and pentose monosaccharides - Explain the formation and breakage of glycosidic bonds - Carry out benedicts test for reducing sugars 1.

Sketch alpha and beta glucose in their simplest form to show their structural differences 2. What type of reaction forms a glycosidic bond? Condensation PLENARY: QUICK QUIZ - Compare alpha and beta glucose molecules - Describe the ring structure and properties of

glucose and ribose - Compare hexose and pentose monosaccharides - Explain the formation and breakage of glycosidic bonds - Carry out benedicts test for reducing sugars 3. Sketch in full the alpha glucose structure 4. What is lactose an example of? Disaccharide

What monosaccharides are sucrose made from? 5. Glucose & Fructose HOMEWORK Write up practical in lab book, must include: method for Reducing Sugar Results for reducing sugar and iodine test

Photos of results EXTENSION: GROUP TASK Make 2 alpha glucose molecule and join them together by a glycosidic bond to make maltose Get your teacher to check your model Join your maltose model with another groups to make amylose

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