Parts and Functions of a Compound Microscope Light
Parts and Functions of a Compound Microscope Light Microscope Simple uses a single lens Compound uses a set of lenses or lens
systems Simple Light Microscope Compound Microscope Mechanical Parts
Used to support and adjust the parts Magnifying Parts Used to enlarge the specimen Illuminating Parts
Used to provide light Mechanical Parts Base Bottommost portion that supports the entire/lower microscope
Pillar Part above the base that supports the other parts Inclination Joint Allows for tilting of the microscope for
convenience of the user Inclination Joint Pillar Base
Arm/Neck Curved/slanted part which is held while carrying the microscope Stage Platform where object to be examined
is placed Stage Clips Secures the specimen to the stage Stage Opening
Body Tube Attached to the arm and bears the lenses Draw Tube Cylindrical structure on top of the body
tube that holds the ocular lenses Draw Tube Body Tube Arm / Neck
Stage Revolving/Rotating Nosepiece Rotating disc where the objectives are attached
Dust Shield Lies atop the nosepiece and keeps dust from settling on the objectives Dust Shield
Revolving Nosepiece Coarse Adjustment Knob Geared to the body tube which elevates or lowers when rotated
bringing the object into approximate focus Fine Adjustment Knob A smaller knob for delicate focusing bringing the object into perfect focus
Coarse Adjustment Knob Fine Adjustment
Knob Condenser Adjustment Knob Elevates and lowers the condenser to regulate the intensity of light
Iris Diaphragm Lever Lever in front of the condenser and which is moved horizontally to open/close the diaphragm Iris
Diaphragm Lever Condenser Adjustment Knob
Illuminating Parts Mirror Located beneath the stage and has concave and plane surfaces to gather and direct light in order to illuminate the object
Electric Lamp A built-in illuminator beneath the stage that may be used if sunlight is not preferred or is not available Mirror /
Electric Lamp Substage Iris Diaphragm Regualtes the amount of light necesaary to
obtain a clearer view of the object Condenser A set of lenses between the mirror and the stage that concentrates light rays on the specimen.
Iris Diaphragm Condenser MAGNIFYING PARTS
Ocular / Eyepiece Another set of lens found on top of the body tube which functions to further magnify the image produced by the objective lenses. It usually ranges from 5x to 15x.
Ocular MAGNIFYING PARTS Objectives Metal cylinders attached below the nosepiece and contains especially ground and polished lenses
LPO / Low Power Objective Gives the lowest magnification, usually 10x HPO / High Power Objective Gives higher magnification usually 40x or 43x
OIO / Oil Immersion Objective Gives the highest magnification, usually 97x or 100x, and is used wet either with cedar wood oil or synthetic oil Objectives
Use of the Compound Microscope Make sure all backpacks are out of the aisles before you get a
microscope! Always carry the microscope with one hand on the Arm and one hand on the Base. Carry it close to your body.
Be gentle. Setting the microscope down on the table roughly could jar lenses and other parts loose. Always start and end with lowest
powered objective. Place the slide on the microscope stage, with the specimen directly over the center of the glass circle on the stage (directly over the light).
If you wear glasses, take them off; if you see only your eyelashes, move closer. If you see a dark line that goes part way across the field of view, try
turning the eyepiece. Use only the Fine adjustment knob when using the HIGH (long) POWER OBJECTIVE. As much as possible, keep both eyes
open to reduce eyestrain. Keep eye slightly above the eyepiece to reduce eyelash interference. If, and ONLY if, you are on LOW POWER, lower the objective lens to
the lowest point, then focus using first the coarse knob, then the fine focus knob. Adjust the Diaphragm as you look through the Eyepiece, and you
will see that MORE detail is visible when you allow in LESS light!
Too much light will give the specimen a washed-out appearance. Once you have it on High
Power remember that you only use the fine focus knob! The High Power Objective (40x) is very close to the slide. Use of the coarse focus knob will scratch the lens, and crack the slide.
MAGNIFICATION The ratio of the original image to the magnified image. RESOLUTION
limiting distance between two points at which they are perceived as distinct from one another. Numerical Aperture
the amount of light that which enters the objective. The larger the NA, the greater the resolving power of the objective. Mounting
Glass Slide - thin flat piece of glass, typically 75 by 25 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Cover Slip
Mounting 1. Gather a thin slice/piece of whatever your specimen is. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw:
2. Place ONE drop of water directly over the specimen. Place the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go.
Staining A technique in microscopy that is used to enhance the image of the specimen. To distinguish structures in cells and tissues
How to Stain a Slide 1. Place one drop of stain on one edge of the coverslip, and the flat edge of a piece of paper towel on the other edge of the coverslip. The paper towel will draw the water out from under the coverslip, and the
cohesion of the water will draw the stain under the coverslip. As soon as the stain has covered the area containing the specimen you are finished. The stain does not need
to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it does. 3. Be sure to wipe off the excess
stain with a paper towel, so you dont end up staining the objective lenses. 4. You are now ready to place the slide on the microscope stage. Be
sure to follow all the instructions as to how to use the microscope. 5. When you have completed your drawings, be sure to wash and dry both the slide and the
Government Procurement (Judicial Review) Act. establishes a new external review process (finally) compliant with international obligations under the GPA and TTP. Legislation passed in October 2018 and comes into force in . April 2019. University of Adelaide
"This is community's data for which I am responsible." earthchem Integrating Data Management for Igneous Geochemistry GEOROC NAVDAT PetDB Common Objective Create a research tool that maximizes application of the geochemical data set.
ICT. 11. Mind set. Environment. Plan. Revising. Revision. Timetable. Exam dates. Early. ... The AQA Anthology and . unseen poetry. How can you help? Reading. Every day- ensure that at least 15 minutes in addition to any revision is spent...
The graph of a parent function is shown. Identify each function which belongs to this same family. Students would benefit from additional practice in identifying equations that belong to the same parent function. The example shown is a rational parent...
Review Monday's lesson. Read "Jack Draws A Bean Stalk" Complete 1 or 2 rotations of Group Practice . Daily Goals: Wednesday. Flex Time Assignment: Choose one illustration method and describe the steps in 5 sentences. Review Tuesday's Lesson.
Fatty acids are stored as neutral lipids, triaclyglycerols (TGs) TGs are hydrophobic, stored in fat cells (adipocytes) Structure of a . triacylglycerol. Prentice Hall c2002. Chapter 9. Structures of glycerophospholipids. Phosphatidylcholine (PC)
Ready to download the document? Go ahead and hit continue!