Molecular interactions between hosts and pathogens Partho Ghosh

Molecular interactions between hosts and pathogens Partho Ghosh

Molecular interactions between hosts and pathogens Partho Ghosh Department of Chemistry & Biochemistry and Section of Molecular Biology University of California, San Diego http://pghosh.ucsd.edu Infectious Diseases at the Molecular Level Pathogens Virulence Factors

Host cells Host Cell Target Establishment of niche in host Subversion of host defenses and immunity Proliferation in host Transmission to other hosts Type III Secretion Needles Human cell

Pathogenic bacterium Kubori et al., Science 280:602 (98) Type III Secretion System (TTSS) Gram-negative Pathogens of mammals Escherichia coli Pseudomonas aeruginosa Salmonella tymphimurium Shigella flexneri Bordetella pertussis Chlamydia trachomatis Yersinia spp.

Pathogens of plants Erwinia amylovora Pseudomonas syringae Ralstonia solanacearum Xanthomonas campestris Pseudomonas aeruginosa Ubiquitous and antibiotic-resistant bacterium Problem for patients with weakened immune

systems Certain strains cause severe and acute infections CDC 90% of severe and acute strains inject the protein ExoU into human cells, results in cell death How does ExoU function? Using genome sequences to get the answer

Phospholipase A2 motifs destroy mammalian cell membranes But, overall identity to phospholipase ~10% Phillips et al. JBC (03) Testing the phospholipase idea: Syringe Loading 106 mammalian cells + ExoU ?

Clarke & McNeil. (1992) J Cell Sci 102:533-41 Si te U o Ex 1 Cell Death

ExoU kills mammalian cells and needs sites 1 and 2 te i S 2 ExoU needs a mammalian cell component ExoU-GFP

Learning what that component is using yeast Yeast without ExoU Yeast making ExoU Cell Death ExoU kills cells but Phospholipase inhibitors block cell death

Inhibitors Will inhibitors stop Pseudomonas? Pseudomonas aeruginosa + inhibitor Cell Death Inhibitors protect against Pseudomonas s as s

a a n n o o U on r m om o m to o x do bi d d E u eu o u hi

e / se In s s w P P P + Current Ann Cai Alicia Gamez Jyothi Kumaran

Case McNamara Jason Miller Rebecca Phillips Catarina Raposo Loren Rodgers UCSD Ed Dennis David Six Lorraine Pillus William Fenical University of Toronto Charlie Boone

Funding W.M. Keck Foundation Cystic Fibrosis Foundation

Recently Viewed Presentations

  • National Electrical Code 2004 - Quia

    National Electrical Code 2004 - Quia

    Branch Circuit. The circuit conductors between the final overcurrent device protecting the circuit and the outlet(s). Such as the last fuse or circuit breaker. Branch circuits are divided into four categories: Appliance. General Purpose. Individual. Multiwire
  • "OUTSOURCING" FOOD & BEVERAGE - National Golf Course ...

    "OUTSOURCING" FOOD & BEVERAGE - National Golf Course ...

    "OUTSOURCING" FOOD & BEVERAGE Disadvantages Less Potential Income Loss of Pricing Control Maybe less "teamwork" Loss of space control for golf events Danger of bad concessionaire "OUTSOURCING" FOOD & BEVERAGE When contracting F&B emphasize the following: Golf Orientation (Golf First)...
  • Formula Mass or Molecular Mass - SJ HS Chemistry

    Formula Mass or Molecular Mass - SJ HS Chemistry

    Chapter 5 5.5 The Mole 5.6 Molar Mass 5.7 Mole Relationships in Chemical Equations 5.8 Mass Calculations for Reactions
  • Hardwoods & Softwoods Wood Characteristics Key words to

    Hardwoods & Softwoods Wood Characteristics Key words to

    Many species of Teak come form non renewable sources Teak is used in Boat building, Garden furniture, (where weather resistance is required) Furniture making, flooring, laboratory benches and decorative veneers. Note: It is best to use an oil finish with...
  • SUNDAY 9th July 2017 Today Mark Baxby will

    SUNDAY 9th July 2017 Today Mark Baxby will

    The 'Chapel A Charity Cricket Match 2017' will take place at Woodhouse CC on Meanwood Road on Thursday 13th July at 6pm. It should be a fun night and all are welcome. Please let James Dey know if you want...
  • Neolithic DBQ - White Plains Middle School

    Neolithic DBQ - White Plains Middle School

    The purpose of writing a DBQ graphic organizer is to develop the basic structure of the WHAP scoring rubric and how it applies to taking 7 documents on a subject and planning for 15 minutes and writing for 45 minutes...
  • Domino's Pizza - NYU

    Domino's Pizza - NYU

    Market Types Awareness Slow, Standard Motivation Relative Size or Fast Cycle Likelihood of Attack Capability First Mover Incentives Speed Competitive Innovation Outcomes Likelihood of Response Sustained Quality Type of Competitive Competitive Competitor Analysis Action Advantage Actor's Reputation Temporary Dependence on...
  • Weathering and Erosion

    Weathering and Erosion

    The rock at a certain location is deeply scratched, and in some places is covered by a layer of unsorted sediment. Which erosional agent was probably responsible for these features?