Dynamic Imaging Based Particle Analysis & The Application

Dynamic Imaging Based Particle Analysis & The Application

Dynamic Imaging Based Particle Analysis & The Application of this Technology for the Analysis of Mammalian Cells Vision Based Particle Sizing Basic Principle CORE & Minicell Benefits

CORE system is designed for laboratory use. Complete system can easily fit in fume hood or on a small bench. System takes advantage of ultra high intensity strobed LED. System power, computer (VCM), pumps and touch-screen monitor all incorporated into one design. System takes advantage of up to 8.9 Megapixel Camera coupled with ultra high resolution optics for measurements down to .5m. Sample size down to .5mL

Software controlled pumps allow for easy sampling. LED Strobed Technology (2 s Pulsewidth) allows for flowrates through analyzer up to 15 fps. Transmitted light microscopy visible to NIR. 8.9 Mega-Pixel CMOS Imaging Sensor

(15 FPS). Measurement capability to .5m Calibration verification using NIST Traceable Polystyrene Microspheres. High speed software with easy to use graphical user interface. 18 L x 16 W x 24 H

Camera Options Resolution Advantage Cell image on left taken with 8.9 Megapixel camera (12m minor diameter cell size) versus image on right taken with standard 2 megapixel ( 12m minor diameter cell size). Zoomed in 100%. 8.9 Megapixel 4x the Resolution of Typical Cameras

Allows for extreme cell size accuracy when measuring very small cells as well as advanced cell classification based on morphology. 2.0 Megapixel Standard camera

While small cells are able to be identified and sized, it can be very difficult to classify different types of cells at this range. Illumination Bright even lighting allows for cells to be easily detected,

measured and classified. Pulsed LED allows for bright transmitted light microscopy without heat. Software advanced flat field correction allows for extremely even background intensity in the most challenging situations. VCM (Vision Control Module) Canty CORE systems come with a high powered VCM built in. VCM has been fully

factory tested and is set up with the following features. I7 Processor (SkyLake - Latest Generation) 20 GB RAM

240 GB Solid State Hard Drive CantyVision Software Pre-Installed and Setup for Plug and Play Use. System Temperature Monitor Watchdog (allows for automated system reset should there be a Windows problem). 4 Gigabit Ethernet Ports (Camera, LED and two additional for LAN connectivity)

CORE Accessories Micro peristaltic pumping system up to 2 pumps running simultaneously Automated cell dilution system using multiple peristaltic pumps fully automated by system software. Automated cell dilution allows for almost unlimited cell concentration analysis without manual dilution prior to running your samples.

CantyVision software identifies cells within the retrieved images through advanced thresholding algorithm. These images are analyzed through pixel counting and the application of a pixel scale factor to provide information on different shape and concentration parameters. Particles are then classified into multiple

classes based on neural classification system. Thousands of cells are fed into a AI machine learning system that then determines viable, necrotic, apoptosis and agglomerated cells and cell density. CantyVision software can output historical data from

multiple runs detailing cell viability, size, concentration and many other factors. Data spanning multiple days/hours can be plotted and compared to give insight into cell growth.

Viable Cells in Classification Window Necrotic Cells in Classification Window Using an advanced machine learning classification system the CantyVision software can determine morphological, color and size differences between various

cell types. Blebs Cell Information Display Window This concludes the Presentation!

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