glutathione-S-transferase (GST) pull down co-purification chromatography, tandem affinity purification (TAP) yeast two hybrid phage display/expression libraries FRET
solution binding- Scatchard analysis Co-Immunoprecipitation Control IP WCE IP A A
Control IP WCE IP A B IP protein A
Resolve Immune Complex by SDS PAGE WesternBlot with Antibody against B Tandem Affinity
Purification (TAP) dvantages Specificity good for complex PTM/localization acks
verification quantitative as sensitive as 2 hyb (for transient) SILAC (Stable Isotope Labeling of Amino-Acid in Cell Cult Yeast Two Hybrid
DNA binding domain hybrid Advantages -sensitivity Activation domain encoded by a library awbacks
ack of specificity alse positives roblems with PTM roblems with localization Interaction Gal1-lacZ (blue
colonies) CHIEN, CT, BARTEL, PL, STERNGLANZ, R, AND FlELDS, S The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA Vol. 88, pp. 9578-9582, November 1991 Fluorescence Resonance Energy Transfer: FRET
Tahoma Times New Roman Times-Roman Times-Italic Spatial Chap4: Spatial Storage and Indexing 4.1 Storage:Disk and Files 4.2 Spatial Indexing 4.3 Trends 4.4 Summary Learning Objectives Physical model in 3 level design? What is a physical data model?
A broader look at culture. Culture is "the collective mixture of differences and similarities that includes for example, individual and family, and community characteristics, values, beliefs, experiences, backgrounds, preferences, and behaviors."
Illustration of Landscape Improvements at the Property Situated at: N. Include compass bearing. Include lot sketch with floor plan. Use plant symbols and common names to identify the selection . and placement of shrubs, trees and other significant features ....
Family CommitmentsThe Great Care Chasm. Society has changed but women assume greater responsibility for childcare and taking care of aging and ill family members which makes it difficult to go away for months of training and/or to relocate.
In this example we did not need all p(n), 0 n k, but we did need p(k) and p(k-1). Note that a proof using weak induction would only be able to assume p(k). Mathematical Induction Rosen 3.3 Basics The Well-Ordering...