Canine Aspergillosis - OMICS Publishing Group

Canine Aspergillosis - OMICS Publishing Group

BROADLY REACTIVE PAN-VIRAL PCR OF CEREBROSPINAL FLUID IN CANINE MENINGOENCEPHALITIS OF UNKNOWN ETIOLOGY The canine meningoencephalitides of unknown etiology (MUE) GME,NME,NLE Histopathologic lesions are similar to those present in human viral meningoencephalitis PCR method has demonstrated that 50-70% of human meningoencephalitides are caused by CNS viral infections. We hypothesize that a subset of canine MUE results from aberrant immune responses following infection of the CNS. Objective : Objective : To determine whether or not nucleic acids from infectious agents can be identified in cerebrospinal fluid (CSF) by applying degenerate viral PCR to 146 CSF samples, collected pre- and/or postmortem from dogs with MUE and control dog. Consensus Degenerate Primers (CODEHOP)

Motif: S I I Q A H N CODEHOP: 5TCC ATC ATC CAG GCC CA C T AA L

C C T T A T C G C TG T 5 Consensus Clamp 3 Degenerate Core

Rose et al., 1998, Nucl Acids Res 26:1628 Viral Families: Herpesviridae, Adenoviridae, Alphaviridae, Picornaviridae, Paramyxoviridae, Polyomaviridae, Flaviviridae, Bunyaviridae, Bornaviridae, Rhabdoviridae, Coronaviridae Pan-Viral PCR Positive Controls Methods DNA and RNA extracted from 146 CSF samples and non-neurological controls by standard methods (Qiagen and Invitrogen) Housekeeping PCR and RT PCR for GAPDH (DNA) and beta-actin to confirm DNA and RNA integrity PCR and RT PCR on ~ 5 l of each sample in 20 various CODEHOP reactions Broadly reactive pan-viral PCR on MUE CSF (146 cases) Coronavirus

Bornavirus Rhabdovirus Alphavirus Herpesvirus Flavivirus Pan-bunyavirus PCR LaCrosse virus Breed CSF from 6/60 (10%) MUE cases positive on panbunyavirus PCR Sequences analysis disclosed 99% homology to LAC Black et al. J Vet Diagn Invest. 1994 Apr;6(2):250-4. Specific LAC PCR underway Developing an Ab to

LaCrosse to perform serology on CSF and serum in dog Shih Tzu Sex Color Weight M/N Wh/ Tan 7.4kg CSF RBC=1963 WBC=12 TP=23.8 Mixed pleocytosis MRI Diagnosis MUE (brain)

normal brain Pug F/S Black 8.25kg Weimaraner F/S Gray 25.8kg RBC=87 WBC=9 TP=17.0 Macro56%,Neut1 8%, Lymph16%, Eos10%

RBC=0 WBC=3 TP=38.7 RBC=38 WBC=26 TP=96.1 Lymph85%, Macro13%, neut2% 9.3kg RBC 69 WBC 20 TP 16.5; 2%% nondeg neut 19% lg mono 79% sm lymphs normal brain MUE (brain) 3.8kg WBC 1 TP

19.5 ;1% nondeg neut 26% lg mono 73% sm lymphs T2W multifocal hyperintense lesions throughout brain MUE (brain) Boxer Boston Terrier Chihuahua M M F/S

Fawn Blk/Wh Wh 37kg T2W hyperintensity cingulate gyrus T2W IM hyperintensity thoracic cord N/D Bunyavirus brain tumor +/meningitis MUE (T/L) MUE (brain) Pan-polyomavirus PCR Merkel Cell

Polyomavirus CSF from 3/60 (5%) MUE cases positive on panpolyomavirus PCR Sequencing (320 bp) shows 98% homology to Merkel Cell Polyomavirus (MCV) Feng et al. Science. 2008 Feb 22;319(5866): 1096-100. IHC on one case negative with human MCV Ab Specific MCV PCR underway CSF antigen testing Labrador Retriever Border Collie Great Dane F/I Chocol ate

26 kg M/N Blk/Wht 18.2 kg M/N RBC=1263 WBC=11 TP=19 16% Lymphs 10%Monos 73%Neuts 1%Eos RBC = 30, WBC = 139 TP 28 mixed pleocytosis RBC 4 WBC 53 TP 56 17% monos 52% eos 31% lymphs Normal cervical spine.

MUE (cervical) Hydrocephalus and syringohydromyelia MUE Syringohydromyelia MUE

Recently Viewed Presentations

  • 第三屆台灣駭客年會 Third Hacks in Taiwan Conference

    第三屆台灣駭客年會 Third Hacks in Taiwan Conference

    第三屆台灣駭客年會 Third Hacks in Taiwan Conference Wayne Huang 黃耀文 [email protected]
  • What about Other Gospels?

    What about Other Gospels?

    Mary is the virgin whom no power defiled. She is a great anathema to the Hebrews, who are the apostles and the apostolic men.… And the Lord would not have said "My Father who is in heaven" unless he had...
  • Magnetism in ultracold Fermi gases and New physics

    Magnetism in ultracold Fermi gases and New physics

    T.L. Ho (2009) Stoner Instability New feature of cold atoms systems: non-adiabatic crossing of Uc Screening of U (Kanamori) occurs on times 1/EF Two timescales in the system: screening and magnetic domain formation Magnetic domain formation takes place on much...
  • Lesson 3: The African Slave Trade UNIT 5:

    Lesson 3: The African Slave Trade UNIT 5:

    European colonists were establishing plantations in North and South America. They wanted slaves to do the hard, undesirable labor. Portugal sold slaves to work on sugar plantations that its colonists had developed in Brazil. Spain also sold slaves to work...
  • MLA 2010 PubMed LinkOut User Meeting

    MLA 2010 PubMed LinkOut User Meeting

    Tuesday, May 25 Medical Library Association Annual Meeting 2010 Washington, D.C. * * * * * * * * * * Agenda LinkOut Updates LinkOut Changes in PubMed Redesign Training Resources and Plan Open Discussion LinkOut Updates Kathy Kwan LinkOut...
  • Seniors: What can I do today to prepare

    Seniors: What can I do today to prepare

    College Checklist. Make a list of colleges.. Junior/Senior Workshop: Tuesday, October 21 @ 6. Check your high school grades and courses.
  • Fundamentals of Game Design, 2nd Edition - Ch 1

    Fundamentals of Game Design, 2nd Edition - Ch 1

    Pygame (like most computing applications) uses additive color models. Colors are determined by three numbers indicating the amount of red, green, and blue light in a pixel. Each value ranges between 0 and 255 (base 10) or between 0x00 and...
  • 5.5 The student will a) find the sum, difference, product ...

    5.5 The student will a) find the sum, difference, product ...

    5.5 The student will a) find the sum, difference, product, and quotient of two numbers expressed as decimals through thousandths (divisors with only one nonzero digit b) create and solve single-step and multistep practical problems involving decimals 5.8 The student...