Brown iGEM

Brown iGEM

A genetic switch Genetic Regulatory Mechanisms in the Synthesis of Proteins Franois Jacob and Jacques Monod. Journal of Molecular Biology (1961) 3: 318-356 Trish Barker: access.ncsa.uiuc.edu/CoverStories/lac/ Gene Synthesis bacteria Source: The Economist 06 Desired gene Desired program Genome Transplantation in Bacteria: Changing One Species to Another Science June 21, , Carole Lartigue 1, John I. Glass 1*, Nina Alperovich 1, Rembert Pieper 1, Prashanth P. Parmar 1, Clyde A. Hutchison III 1, Hamilton O. Smith 1, J. Craig Venter 1 Issues 1. Cost & length of DNA synthesis

2. How to boot the system 3. How to design and build it Synthetic Biology A new foundational discipline to deal with the coming technology An effort to design and build standardized, integrated biological systems 10 years time You can design new systems Build them quickly and cheaply What are you going to build? Synthetic Biology @ Brown Brown iGEM International Genetically Engineered Machines iGEM Video Engineering biology with

standard parts Registry of Standard Biological Parts Parts Devices Systems The Process Design Build Model 7 Brown Undergraduates Biomedical Engineering Biology Biophysics Chemical Engineering Computational Biology iGEM Faculty Tayhas Palmore

Biomedical Engineering Alex Brodsky Molecular Biology, Cell Biology and Biochemistry Karen Haberstroh Biomedical Engineering Mark Johnson Molecular Biology, Cell Biology and Biochemistry Jeffrey Morgan Pharmacology, Physiology & Biotechnology. Nicola Neretti Physics Ben Raphael

Center for Computational Biology Sherief Reda Electrical Engineering Suzanne Sindi Applied Math Jay Tang Physics David Targan Physics Gary Wessel Molecular Biology, Cell Biology and Biochemistry Support

UTRA grant & departments Lab space in Multi Disciplinary Lab Equipment sponsorship Brown iGEM Two projects being built with biological parts Lead-detector Tri-stable Switch Lead Detector The Abstract System Amplifier Lead Promoter Fluorescen t Protein So how will this system work in the cell?

Promoter (always on) Lead Promoter Message Promoter Lead Receptor Message Producer Message Producer NO LEAD Message Receptor

Transcription factors are constitutively made by the first promoter. GFP These proteins are poised to activate the Lead Detector promoter and Message Receiver promoter upon addition of lead. Promoter (always on) Lead Promoter Lead Receptor Message

Receptor Message Producer Lead turns on Detector promoter + Message Promoter Message Producer GFP Fluorescent Protein Output Tri-Stable Switch Bistable Switch

Here is how a bistable switch should work: Continuous production of A or B without additional inducer A B Tristable Switch A two state switch is possible. We want a stable switch with three distinct inducible states. A C B

Tristable Switch Design Pathway A pTet LacI AraC AraC TetR Pathway B pLac Pathway C pAra LacI TetR Uses for a Multi-stable Switch Tissue Engineering

Drug Delivery Simple Circuits Applications of synthetic biology Programmed cell-cell communication S. Basu, et al A synthetic multicellular system for programmed pattern formation Nature 2005, 434, 1130. Engineer sender and receiver cells Programmed pattern formation Programmed cell-cell communication applied to Stem Cells? Cell fate regulators Aim: engineer tissues from stem cells Approach: drive differentiation of stems cells down a specific pathway using engineered sender cells

Metabolic Engineering hijacking a cell to produce antimalarial precursor D-K. Ro et al. Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 2006, 440, 940 Population at risk: 3.2 Billion Endemic countries: 107 # of infections annually: 300-500 Million # of deaths annually: 1-3 Million Cost per adult course: $2.40 Biofuels Challenge: petroleum production peaks in 10-30 yr, declines until resources are exhausted; 1 gal of gasoline and diesel = 20 lbs CO2 (7 tons/vehicle/yr) Discover microbes capable of pretreatment and/or hydrolysis of lignocellulosic material Engineer (i.e., synthetic biology) microorganisms to convert the monomer products of deconstruction into fuels BP, Synthetic Genomics-JCVI, Amyris Biotechnologies-UCB Synthetic Biology Funding

The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields. Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students. Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world. SynBERC is a NSF Engineering Research Center The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems

in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields. Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students. Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world. SynBERC is a NSF Engineering Research Center The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field.

Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields. Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students. Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world. SynBERC is a NSF Engineering Research Center The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies

through its relationships with industry in synthetic biology-related fields. Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students. Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world. SynBERC is a NSF Engineering Research Center The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields. Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide

general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students. Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal on ways that economic, political, and Cal emphasis to be hub cultural forces may condition the development of synthetic biology and on waysfothat synthetic may r stu dy of altbiology e r n G a ro te significantly inform human security, health, and welfare throughRitheupnew fuel headobjects ed by UC that it brings into the world. ck DelVec

chio, Mar kM Berkeley Thursday artin, Chronicle Staff Writwersins $500 million grant fro , February m BP 1, 2007 SynBERC is a NSF Engineering Research Center The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to layL.the foundation for this nascent field. By MATTHEW WALD Published: June 26, 2007 Broader impacts

WASHINGTON, June 25 The Energy Department is creating three bioenergy research centers to Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, find pharmaceutical, new ways to turn plants into fuel. as well as suppliers of genetic tools and custom DNA synthesis companies and chemical industries, Thethrough three centers, which with the industry department described as threefields. start-up companies with $125 million each in its relationships in synthetic biology-related capital, will beand in training: Oak Ridge,

Tenn.;will Madison, and near Berkeley, Calif.and They will involve Education SynBERC educate aWis.; new cadre of synthetic biologists biological engineersnumerous capable of designingnational biological laboratories parts and usefuland biological systems. Finally, SynBERCs education program

will provide universities, private companies. The goal of the centers, which are to be general information on synthetic biology the general public, in-depth offerings public policy professionals, and announced on Tuesday, is to bring newfortechnologies to market within fivefor years. motivational information on opportunities in higher education for K-12 students.

The new approach supports President Bushs goal of reducing gasoline consumption by 20 percent in 10 Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of years. synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and Cal to e hways ub fothat cultural forces may condition the development of synthetic biology and bon synthetic may r stu dy of altbiology e r n G a ro te significantly inform human security, health, and welfare throughRitheupnew that it brings into the world. fuel headobjects ed b

ck DelVec y UC Berke chio, Mar ley wins k Martin, Chronicle Staff Writ ers Thursday ,F ebruary 1 , SynBERC is a NSF Engineering Research Center $500 milli on grant 2007

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ls mor Department is creating three bioenergy research centers to kilEnergy WASHINGTON, Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, find pharmaceutical, new ways to turn plants into fuel. as well as suppliers of genetic tools and custom DNA synthesis companies and chemical industries, Thethrough three centers, which with the industry department described as threefields. start-up companies with $125 million each in its relationships in synthetic biology-related capital, will beand

in training: Oak Ridge, Tenn.;will Madison, and near Berkeley, Calif.and They will involve Education SynBERC educate aWis.; new cadre of synthetic biologists biological engineersnumerous capable of designingnational biological laboratories parts and usefuland biological systems. Finally, SynBERCs

education program will provide universities, private companies. The goal of the centers, which are to be general information on synthetic biology the general public, in-depth offerings public policy professionals, and announced on Tuesday, is to bring newfortechnologies to market within fivefor

years. motivational information on opportunities in higher education for K-12 students. The new approach supports President Bushs goal of reducing gasoline consumption by 20 percent in 10 Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of years. synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and Cal to e hways ub fothat cultural forces may condition the development of synthetic biology and bon synthetic may r stu dy of altbiology e r n G a ro te significantly inform human security, health, and welfare throughRitheupnew that it brings into the world. fuel

headobjects ed b ck DelVec y UC Berke chio, Mar ley wins k Martin, Chronicle Staff Writ ers Thursday ,F ebruary 1 , SynBERC is a NSF Engineering Research Center $500 milli on grant

2007 from BP Department of Health and Human Services Participating Organizations National Institutes of Health (NIH), (http://www.nih.gov) Components of Participating Organizations National Heart, Lung, and Blood Institute (NHLBI) (http://www.nhlbi.nih.gov) National Cancer Institute (NCI), (http://www.nci.nih.gov) National Institute on Aging (NIA) (http://www.nia.nih.gov) National Institute of Biomedical Imaging and Bioengineering (NIBIB) (http://www.nibib.nih.gov) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (http://www.niddk.nih.gov) Title: Bioengineering and Obesity (R01) Announcement Type New Update: The following update relating to this announcement has been issued: April 23, 2007 - See Notice (NOT-HL-07-112) The purpose of this notice is to update the contact information. NOTICE: Applications submitted in response to this Funding Opportunity Announcement (FOA) for Federal assistance must be submitted electronically through Grants.gov (http://www.grants.gov) using the SF424 Research and Related (R&R) forms and the SF424 (R&R) Application Guide APPLICATIONS MAY NOT BE SUBMITTED IN PAPER FORMAT. This FOA must be read in conjunction with the application guidelines included with this announcement in Grants.gov

/Apply for Grants (hereafter called Grants.gov/Apply). A registration process is necessary before submission and applicants are highly encouraged to start the process at least four weeks prior to the grant submission date. See Section IV. Program Announcement (PA) Number: PA-07-354 Executive Summary Purpose This Funding Opportunity Announcement (FOA) issued by the National Heart, Lung, and Blood Institute, National Institutes of Health, solicits Research Project Grant (R01) applications from institutions/organizations that propose to solicit applications to develop and validate new and innovative engineering approaches to address clinical problems related to energy balance, intake, and expenditure. Novel sensors, devices, imaging, and other technologies, including technologies to detect biochemical markers of energy balance are expected to be developed and evaluated by collaborating engineers, physical scientists, mathematicians, and scientists from other relevant disciplines with expertise in obesity and nutrition. Mechanism of Support. This FOA will utilize the NIH Research Project Grant (R01) award mechanism and runs in parallel with an FOA of identical scientific scope, RFA-HL-07-007, that solicits applications under the R21 mechanism and FOA number(s) as appropriate. Funds Available and Anticipated Number of Awards. Awards issued under this FOA are contingent upon the availability of funds and the submission of a sufficient number of meritorious applications. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size and duration of each award will also vary. The total amount awarded and the number of awards will depend upon the mechanism numbers, quality, duration, and costs of the applications received. Eligible Institutions/Organizations. Public/State Controlled Institution of Higher Education; Private Institution of Higher Education; Nonprofit with 501(c)(3) IRS Status (Other than Institution of Higher Education); Nonprofit without 501(c)(3) IRS Status (Other than Institution of Higher Education); Small Business; For-Profit Organization (Other than Small Business); State Government; U.S. Territory or Possession; Indian/Native American Tribal Government (Federally Recognized); Indian/Native American Tribal Government (Other than Federally Recognized); Indian/Native American Tribally Designated Organization; Non-domestic (non-U.S.) Entity (Foreign Organization); Hispanic-serving Institution; Historically Black Colleges and Universities (HBCUs); Tribally Controlled Colleges and Universities (TCCUs); Alaska Native and Native Hawaiian Serving Institutions; Regional

Organization. Eligible Project Directors/Principal Investigators (PDs/PIs). Individuals with the skills, knowledge, and resources necessary to carry out the proposed research are invited to work with their institution/organization to develop an application for support. Individuals from underrepresented racial and ethnic groups as well as individuals with disabilities are always encouraged to apply for NIH support. Number of Applications. Applicants may submit more than one application, provided each application is scientifically distinct. Renewals and Resubmissions. Applications can be renewed by competing for additional project periods. Applicants may submit a resubmission application, but such application must include an Introduction addressing the previous peer review critique (Summary Statement).. Number of PDs/PIs. More than one PD/PI, or multiple PDs/PIs, may be designated on the application. Application Materials. See Section IV.1 for application materials. General Information. For general information on SF424 (R&R) Application and Electronic Submission, see these Web sites: SF424 (R&R) Application and Electronic Submission Information: http://grants.nih.gov/grants/funding/424/index.htm General information on Electronic Submission of Grant Applications: http://era.nih.gov/ElectronicReceipt/ Hearing Impaired. Telecommunications for the hearing impaired is available at: TTY 301-451-0088 . Executive Summary Purpose This Funding Opportunity Announcement (FOA) issued by the National Heart, Lung, and Blood Institute, National Institutes of Health, solicits Research Project Grant (R01) applications from institutions/organizations that propose to solicit applications to develop and validate new and innovative engineering approaches to address clinical problems related to energy balance, intake, and expenditure. Novel sensors, devices, imaging, and other technologies, including technologies to detect biochemical markers of energy balance are expected to be developed and evaluated by collaborating engineers, physical scientists, mathematicians, and scientists from other relevant disciplines with expertise in obesity and nutrition. Mechanism of Support. This FOA will utilize the NIH Research Project Grant (R01) award mechanism and runs in parallel with an FOA of identical scientific scope, RFA-HL-07-007, that solicits applications under the R21 mechanism and FOA number(s) as appropriate. Funds Available and Anticipated Number of Awards. Awards issued under this FOA are contingent upon the availability of funds and the

submission of a sufficient number of meritorious applications. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size and duration of each award will also vary. The total amount awarded and the number of awards will depend upon the mechanism numbers, quality, duration, and costs of the applications received. Eligible Institutions/Organizations. Public/State Controlled Institution of Higher Education; Private Institution of Higher Education; Nonprofit with 501(c)(3) IRS Status (Other than Institution of Higher Education); Nonprofit without 501(c)(3) IRS Status (Other than Institution of Higher Education); Small Business; For-Profit Organization (Other than Small Business); State Government; U.S. Territory or Possession; Indian/Native American Tribal Government (Federally Recognized); Indian/Native American Tribal Government (Other than Federally Recognized); Indian/Native American Tribally Designated Organization; Non-domestic (non-U.S.) Entity (Foreign Organization); Hispanic-serving Institution; Historically Black Colleges and Universities (HBCUs); Tribally Controlled Colleges and Universities (TCCUs); Alaska Native and Native Hawaiian Serving Institutions; Regional Organization. Eligible Project Directors/Principal Investigators (PDs/PIs). Individuals with the skills, knowledge, and resources necessary to carry out the proposed research are invited to work with their institution/organization to develop an application for support. Individuals from underrepresented racial and ethnic groups as well as individuals with disabilities are always encouraged to apply for NIH support. Number of Applications. Applicants may submit more than one application, provided each application is scientifically distinct. Renewals and Resubmissions. Applications can be renewed by competing for additional project periods. Applicants may submit a resubmission application, but such application must include an Introduction addressing the previous peer review critique (Summary Statement).. Number of PDs/PIs. More than one PD/PI, or multiple PDs/PIs, may be designated on the application. Application Materials. See Section IV.1 for application materials. General Information. For general information on SF424 (R&R) Application and Electronic Submission, see these Web sites: SF424 (R&R) Application and Electronic Submission Information: http://grants.nih.gov/grants/funding/424/index.htm General information on Electronic Submission of Grant Applications: http://era.nih.gov/ElectronicReceipt/ Hearing Impaired. Telecommunications for the hearing impaired is available at: TTY 301-451-0088 . Executive Summary

Purpose This Funding Opportunity Announcement (FOA) issued by the National Heart, Lung, and Blood Institute, National Institutes of Health, solicits Research Project Grant (R01) applications from institutions/organizations that propose to solicit applications to develop and validate new and innovative engineering approaches to address clinical problems related to energy balance, intake, and expenditure. Novel sensors, devices, imaging, and other technologies, including technologies to detect biochemical markers of energy balance are expected to be developed and evaluated by collaborating engineers, physical scientists, mathematicians, and scientists from other relevant disciplines with expertise in obesity and nutrition. Mechanism of Support. This FOA will utilize the NIH Research Project Grant (R01) award mechanism and runs in parallel with an FOA of identical scientific scope, RFA-HL-07-007, that solicits applications under the R21 mechanism and FOA number(s) as appropriate. Funds Available and Anticipated Number of Awards. Awards issued under this FOA are contingent upon the availability of funds and the submission of a sufficient number of meritorious applications. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size and duration of each award will also vary. The total amount awarded and the number of awards will depend upon the mechanism numbers, quality, duration, and costs of the applications received. Eligible Institutions/Organizations. Public/State Controlled Institution of Higher Education; Private Institution of Higher Education; Nonprofit with 501(c)(3) IRS Status (Other than Institution of Higher Education); Nonprofit without 501(c)(3) IRS Status (Other than Institution of Higher Education); Small Business; For-Profit Organization (Other than Small Business); State Government; U.S. Territory or Possession; Indian/Native American Tribal Government (Federally Recognized); Indian/Native American Tribal Government (Other than Federally Recognized); Indian/Native American Tribally Designated Organization; Non-domestic (non-U.S.) Entity (Foreign Organization); Hispanic-serving Institution; Historically Black Colleges and Universities (HBCUs); Tribally Controlled Colleges and Universities (TCCUs); Alaska Native and Native Hawaiian Serving Institutions; Regional Organization. Eligible Project Directors/Principal Investigators (PDs/PIs). Individuals with the skills, knowledge, and resources necessary to carry out the proposed research are invited to work with their institution/organization to develop an application for support. Individuals from underrepresented racial and ethnic groups as well as individuals with disabilities are always encouraged to apply for NIH support. Number of Applications. Applicants may submit more than one application, provided each application is scientifically distinct. Renewals and Resubmissions. Applications can be renewed by competing for additional project periods. Applicants may submit a resubmission

application, but such application must include an Introduction addressing the previous peer review critique (Summary Statement).. Number of PDs/PIs. More than one PD/PI, or multiple PDs/PIs, may be designated on the application. Application Materials. See Section IV.1 for application materials. General Information. For general information on SF424 (R&R) Application and Electronic Submission, see these Web sites: SF424 (R&R) Application and Electronic Submission Information: http://grants.nih.gov/grants/funding/424/index.htm General information on Electronic Submission of Grant Applications: http://era.nih.gov/ElectronicReceipt/ Hearing Impaired. Telecommunications for the hearing impaired is available at: TTY 301-451-0088 . Recent faculty searches include: CalTech, MIT, Emory, UC Davis November 24, 2005 Key people, institutes & companies BU: Jim Collins, Tim Gardner, Caltech: Francis Arnold, Michael Elowitz, Christina Smolke, Havard: George Church, Pam Silver MIT

UC Berkeley Adam Arkin, Jay Keasling UC Berkeley Adam Arkin, Jay Keasling UT Austin Andy Ellington Edward Marcotte Drew Endy, Tom Knight, Alexander van Oudenaarden Princeton Ron Weiss, UCSF Wendell Lim Chris Voigt Should Brown pursue synthetic biology as part of its academic plan?

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